Department of Plant Pathology
WELCOME TO THE
Disease Clinic

The Plant Disease Clinic is a service laboratory situated within the Department of Plant Pathology at the University of Stellenbosch. The clinic started its activities in October 2000, and specialists in the field of plant pathology, diagnose problems on the samples received. We diagnose all types of fungal or bacterial diseases on various crops, including trees, shrubs, vegetables, fruit, ornamentals, etc. Virus diseases and insect identifications are also managed by consultants from Vironostix and the Department of Conservation Ecology and Entomology.  Leaf and soil samples for nutritional shortages and nematode analysis can, on request, be sent to experts in that particular field.  Diagnosis can take between one and 2 weeks to be completed, although new diseases require additional pathogenicity tests, which will take much longer.  Each sample submitted will receive a written diagnostic report, explaining the findings, and listing any available control recommendations.

When sending a sample to the Plant Disease Clinic, please include the relevant Diagnostic Form.  Follow our collection and submission tips when submitting samples. Samples that are improperly collected, packed, and/or shipped and are received in a poor condition are very difficult to diagnose.

We aim to provide fast, but accurate diagnoses and always seek new methods for minimising diagnoses time. The digital camera, used conventionally or through microscope lenses, has become a useful diagnostic aid. It enables us to forward digital images of pathogen structures to international experts for verification. Experience gained through work done for the private sector is shared with producers. We also provide valuable practical training for under- and postgraduate students.

Ms Elaine Basson
Technical Officer
Ms Doré de Villiers
Technical Officer
Miss Sonja Coertze
First Technical Officer / Disease Clinic Manager
Mrs Brenda de Wee
Assistant A
Operation Hours

The clinic is open 07:30 till 16:00, Mondays to Fridays.

Courier address

Plant Disease Clinic
Paul vd Bijl
Suidwal
Welgevallen
Stellenbosch
7600

Postal address

Disease Clinic
Stellenbosch University
Private Bag X1
Matieland
7602

Services

The Plant Disease Clinic accepts the submission of problematic plant material and soil samples to be analysed for issues such as phytopathogenic bacteria, fungi, fungal-like organisms, viruses and insects.  The Clinic aims to provide fast, but accurate diagnoses.  Results are normally provided within one to two weeks (depending on the problem at hand).  A proper diagnosis is very important to manage plant diseases.

Plant Diseases: Standard analyses include identification of most plant pathogenic fungi and bacteria, as well as cultural and physiological conditions that are apparent and may be responsible for plant problems.  If identification of genus and species is required of fungal and bacterial isolates, the Disease Clinic uses various molecular techniques if available.  A pure culture is required to perform this procedure with some incubation time.  Please be aware that this process may take an additional 5-7 days to complete (as well as an extra fee).  The Clinic also uses ELISA-based immunostrips, if available, for the detection of certain bacteria.

Virus Diseases:  The Disease Clinic also receives plants to be tested for viruses.  This service is sub-contracted to Vironostix (Department of Genetics).

Insect and mite identification:  The Disease Clinic also offers a service for insect and mite identifications, with the focus on fruit pests in the Western Cape Province.  This service is sub-contracted to consultants from the Department of Conservation Ecology and Entomology.  Specimens are preserved where possible, accessioned and entered into a database for future reference. Where necessary, samples are sent to experts for further processing.

Data History

Statistics on samples from the past couple of years will soon be available.

Submitting plant specimens

When collecting samples for analysis, please keep a few things in mind:

Examine the plant carefully. Symptoms may appear at some distance from the actual site of infection. Look for any irregularities or injuries on the lower portion of the plant.

Collect a sample that is representative of the condition in question.
It is important that you try to collect the sample prior to any pesticide applications – once pesticides have been applied, it may be difficult to obtain an accurate diagnosis.

The sample to be submitted should, when possible, contain all parts of the plant.
Include enough material showing the full range of development of the disease, from healthy to maximum disease development. Collect the sample from an area that shows early development of the problem, as areas, which contain mostly dead plant material, often contain a number of secondary organisms which complicates the identification of the primary pathogen.  Try NOT to send dead plants.

Wrap samples in dry paper towelling, paper bags or clean newspaper (do NOT add water) and pack loosely in a plastic bag to prevent excessive drying.

Package all specimens securely in a sturdy container (Styrofoam container or cardboard boxes) to prevent crushing.  Packing material such as newspaper can be added to prevent damage during transit.

Send only freshly collected samples and use the most rapid transit method. Diagnosis can be difficult, if not impossible, when delays result in extensive deterioration of the sample.

If the sample cannot be send to us promptly, keep it refrigerated or out of direct sunlight and/or heat.

Hints for specific plant material:

1. Fleshy fruits, roots, and tubers – wrap enough clean, absorbent material such as paper towelling to absorb any leakage. DO NOT place samples in plastic bags.

2. Woody plants – for smaller plants submit an entire plant. Enclose the roots and soil with a plastic bag. Canker, dieback etc. on trees or shrubs – cut the branch several centimetres beyond each end of the affected area, so as to include some healthy wood. Dead, deteriorating branches are often worthless as samples.

3. Turf – lift a section of the turf so that healthy grass, along with the margin and the central parts of the affected area, is included in the sample. Enclose the entire sample in a plastic zipper bag (do not add water). When collecting samples from more than one area, use separate bags.

4. Garden plants, green house plants – dig up (do not pull) complete plants, leaving roots and soil intact. Enclose the roots and soil with a plastic bag.  Do not wrap the stem and leaves in plastic.

5. Virus testing – Collect symptomatic leaves, stems or the entire plant and place in a plastic bag.  Do not let the leaves dry out, but do not add extra water.  Leaves can be pressed between layers of paper towelling and then put in zipper plastic bag.

When submitting samples for diagnosis, please keep the following in mind:

A submitted sample must be of good quality and quantity and be submitted with the relevant completed Disease Diagnosis form (available on our Web site)

Provide as much information on the form(s) as possible to ensure an accurate diagnosis of the problem.

Be sure you include the form with your sample. You may also want to keep one copy of the form for your records.

For faster response, include your e-mail address and indicate how you would prefer to receive the results.

Samples are typically processed on a first come, first served basis.

A report (results and control measures) will be e-mailed, faxed or posted as soon as the results are finalised.

An invoice will be send with the report or soon thereafter.

Courier the sample as fast as possible. Possible couriers include:
Sun Courier, XPS, DHL, TNT.

NB:
There is no courier delivery at the University of Stellenbosch over weekends or on public holidays. To ensure your sample get processed as soon as possible, collect and send samples to reach us not later than the Friday morning of a regular week. During weeks with public holidays, please call in advance to make arrangements.
Please feel free to call the clinic with any questions prior to your sample submission.

Submitting Insect Specimens

Collecting samples:

Collect fresh material into a crush-proof container and, if possible, freeze the specimen as soon as possible.

Care should be taken to package the insects so they arrive intact.  Do not tape insects to paper or place them loose in envelopes.

Label with the date and place of collection and the name of the collector. Supply any biological information, for example the host plant etc…

Submit the sample as soon as possible.

Correct handling of samples will ensure that a more accurate identification can be made.

Please complete an insect submission form along with the specimen, which will ensure that you receive prompt feedback.

Popular & Scientific Publications
  1. McLeod, A., Masimba, T., Jensen, T., Serfontein, K. & Coertze, S.  2017.  Evaluating spray programs for managing copper resistant Pseudomonas syringae pv. tomato populations on tomato in the Limpopo region of South Africa.  Crop Protection 102: 32-42.
  2. Jensen, T., Beukes, I., Mostert, L. & Coertze, S.  2017.  First report of white rust on Lampranthus sp. caused by Albugo trianthemae in South Africa.  Plant Disease 101 (1): 260.
  3. Beukes, I., Jensen, T. & Coertze, S.  2016.  First report of Ramularia collo-cygni infecting barley in South Africa.  Journal of Plant Pathology 98 (2): 376.
  4. Beukes, I., Jensen, T. & Coertze, S.  2016.  The identification of diseases on wheat and barley grown in the Western Cape in 2015.  SA Grain April: 64-67.
  5. Coertze, S., Jensen, T., Kotzé, T.N. & McLeod, A.  2015.  Establishing the status of Clavibacter michiganensis subsp. insidiosus in lucerne in South Africa.  Journal of Plant Pathology 97 (2): 283-290.
  6. Mostert, L., Bester, W., Jensen, T., Coertze, S., Van Hoorn, A., Le Roux, J., Retief, E., Wood, A. & Aime, M.C.  2010.  First report of leaf rust of blueberry caused by Thekopsora minima on vaccinium corymbosum in the Western Cape, South Africa.  Plant Disease 94: 478.
  7. Mostert, L., Bester, W., Coertze, S. & Wood, A.  2008.  First report of daylily rust caused by Puccinia hemerocallidis in the Western Cape Province of South Africa.  Plant Disease 92 (7): 1133.
  8. McLeod, A. & Coertze, S.  2007.  First report of Phytophthora cryptogea on Osteospermum spp. in South Africa.  Plant Disease 91: 322.
  9. McLeod, A., Coertze, S. & Mostert, L.  2006.  First report of a Peronospora species on sweet basil in South Africa.  Plant Disease 90: 1115.
  10. McLeod, A. & Coertze, S.  2006.  First report of Phytophthora infestans on Petunia x hybrida in South Africa.  Plant Disease 90: 1550.
  11. Swart, L. & Van Niekerk, J.M.  2003.  First record of Fusarium oxysporum f.sp. basilici occurring on sweet basil in South Africa.  Australasian Plant Pathology 32: 125-126.
  12. Swart, L. & Coertze, S.  2002. First record of Botrytis flower blight, caused by Botrytis cinerea, on Geraldton waxflower in South Africa.  Plant Disease 86: 440.
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